论文：Targeting efficiency of PEG-immunoliposome-conjugated antibodies at PEG terminals小说家：Kazuo Maruyama a, Tomoko Takizawa a, Nobuya Takahashi a, Toshiaki Tagawa b, Kazuhiro Nagaike b, Motoharu Iwatsuru论文资料地址：//www.sciencedirect.com/science/article/abs/pii/S0169409X96004632

摘要：

We have developed a new type of PEG-immunoliposome carrying monoclonal antibodies or their fragments (F(ab′)2, Fab′) at the distal ends of the PEG chains (Type C). Distearoylphosphatidylethanolamine derivatives of PEG with car☐yl group (DSPE-PEG-COOH) or dipalmitoyl phosphatidylethanolamine derivatives of PEG with maleimidyl group (DPPE-PEG-Mal) at the PEG terminal were newly synthesized. Small unilamellar liposomes (90–130 nm in diameter) were prepared from distearoyl phosphatidylcholine and cholesterol (2:1, m/m) containing 6 mol% of DSPE-PEG-COOH or DPPE-PEG-Mal. To target to the vascular endothelial lung surface as a model accessible site, 34A antibody, which is highly specific to mouse pulmonary endothelial cells, was conjugated to PEG-liposome (34A-Type C). The degree of lung binding of 34A-Type C in BALB/c mice was significantly higher than that of the 34A-Type A which is an ordinary type immunoliposome (without PEG derivatives). To target to the solid tumor tissue as a model of the less accessible site, 21B2 antibody which is anti-human CEA and its Fab′ fragment were used. 

开拓好几个种当下的PEG天然免疫脂质体，在PEG链的远端带入单克隆抗原或其段落（F（ab′）2，Fab′）（C型）。聚乙二醇二硬脂酰磷脂酰酒精胺衍化物☐新炼制了PEG末段中带马来酰亚胺基的烷基（DSPE-PEG-COOH）或二棕榈酰磷脂酰酒精胺衍化物（DPPE-PEG-Mal）。由具有6mol%DSPE-PEG-COOH或DPPE-PEG-Mal的二硬脂酰磷脂酰胆碱和热量（2:1，m/m）分离纯化了小编织成单层脂质体（直径约90-130nm）。要为靶向疗法微血管内皮肺面为模板可及位点，将对小鼠肺内皮神经元相对高度特喜欢的人的34A表面抗原与PEG脂质体（34A C型）紧密联系。BALB/C小鼠中34A C型的肺结合在一起系数比较突出大于常见型免役脂质体（不含有PEG繁衍物）34A A型。为了能靶向疗法三维线瘤组织安排当做很难将近局部的类别，在使用了抗人CEA的21B2免疫抗体简答Fab′段落。有关系推存：mPEG-di-Glutamic AcidmPEG-FAmPEG-PEI(25K)mPEG-SBAmPEG-PCL(3K)mPEG-SS-C30mPEG-PCL(10K)mPEG-PCL(12K)mPEG-AlkenemPEG-DFmPEG-DBCOmPEG-DAmPEG-DansylmPEG-Ts 这一篇文章的内容由来几大类论文期刊或学术论文，深表歉意侵权案请联系起来企业册除！