医学文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy著者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie资料链接搜索：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801引言：1H NMR was determined to confirm the synthesis of FA-PEG-PEI (the spectrum and relevant chemical structural formula were shown in Figure 1). The characteristic peaks from 2.0 to 3.0 ppm (k, j) were attributed to -CH2CH2NH- group of PEI, the peaks at 3.66 ppm (h) were referred to -CH2CH2O- group of PEG, and the peaks from 6.5 to 9.0 ppm (a, b, c) were related to the H protons of benzene ring in FA [25]. The data suggested a successful combination of FA-PEG-PEI.The possible functional groups responsible for the synthesis of FA-PEG-PEI were further confirmed by FT-IR spectroscopy. Meanwhile, the FT-IR spectra of FA, NH2-PEG-OH, and NH2-PEG-COOH were also examined. The terminal hydroxyl groups of NH2-PEG-OH were of carboxylation and then combined with the amino groups of PEI via amidation reaction. As shown in Figure 2, the FT-IR spectra of terminal hydroxyl groups of (b) NH2-PEG-OH showed the presence of stretching vibration peak of -OH at 3440 cm−1, which shifted to a new stretching vibration peak of -C=O at 1629 cm−1 in (c) NH2-PEG-COOH after carboxylation. The FT-IR spectra of (d) FA-PEG-PEI displayed a broad peak at 3350–3250 cm−1, which refers to -NH2 and -NH- groups of PEI. The peaks at 1606–1460 cm−1 correspond to the benzene ring of FA. In addition, the peak at 1110 cm−1 is attributed to the symmetrical stretching vibration of -C-O-C- group in PEG, which declares the conjugation of FA-PEG-PEI.

分析1H NMR以查证FA-PEG-PEI的合出。共同点顶值在2.0到3.0中 ppm（k，j）归因于PEI的-CH2CH2NH-基团，顶值在3.66 ppm（h）是说 PEG的-CH2CH2O-基团，峰在6.5至9.0中 ppm（a，b，c）与FA中苯环的H质子有关系。数据资料阐明，FA-PEG-PEI的组合式是成就的。FT-IR光谱分析分析进1步印证了会提供FA-PEG-PEI分解成的官能团。互相，还学习了FA、NH2-PEG-OH和NH2-PEG-COOH的傅里叶改变红外光谱分析分析。NH2-PEG-OH的未端羟基被羧化，而后根据酰胺化体现与PEI的氨基紧密结合。如同2下图，（b）NH2-PEG-OH未端羟基的FT-IR光谱分析仪出现，在3440处具备-OH的折叠产生振荡峰 cm-1，在1629处移动式到新的拉伸弹簧产生振荡峰-C=O 羧化后，在（c）NH2-PEG-COOH添加入cm-1。（d）FA-PEG-PEI的FT-IR光谱分析仪在3350-3250处出现打了个个宽峰 cm-1数字代表PEI的-NH2和-NH-基团。1606年至1460年的顶值 cm-1代表于FA的苯环。显然，1110处的峰 cm-1归因于PEG中-C-O-C-基团的相对性折叠产生振荡，这声明了FA-PEG-PEI的共轭。涉及安利：HZ-PEG-OHPhthalimide-PEG-OHPLL-PEG-OHPLGA(10K)-PEG-OHSilane-PEG-OHOH-PEG-PAOH-PEG-SSOH-PEG-SGLA-PEG-OH以内句子信息内容主要来源各种类型期刊杂志或论文资料，知悉图片侵权请联系起来我去除！