文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

A poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carboxyl (PLGA-PEG-COOH) and gelatin-based microparticles (MPs) dual delivery system for release of bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP-2 and IGF-1-loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP-2 and IGF-1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA-PEG-COOH MPs is in the range of 64.78% to 76.11%. E-beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E-beam sterilization decreases the bioactivity of growth factors in MPs by ≈22%. BMP-2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF-1 from the PLGA-PEG-COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA-PEG-COOH MPs-based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors-loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.

设计好几个种使用于挥发骨体型遭受淀粉酶-2（BMP-2）和胰岛素样的生长分子-1（IGF-1）的聚乳酸-聚乙二醇-羧基（PLGA-PEG-COOH）和明胶基粒子（MP）双递送体系。递送体系的显著特点就在其体型、负载电阻的能力、包封工作效率和挥发能学。理论研究了电子技术束（EB）过滤除菌对过载BMP-2和IGF-1的MP的影晌简述菌物学效果。开展了BMP-2和IGF-1的受控多重缓解压力对之间充质干体细胞（MSCs）成骨的一体化的作用。

生长因子在明胶和PLGA-PEG-COOH MP中的包封效率在64.78%至76.11%之间。电子束灭菌的生长因子递送系统能有效显著促进MSCs的成骨，尽管电子束灭菌会使MP中生长因子的生物活性降低约22%。明胶MPs/PEG水凝胶的BMP-2释放行为比PLGA-PEG-COOH MPs/PEG凝胶的IGF-1释放行为（27.3%）更快（52.7%）。

报告显示，能够明胶和PLGA-PEG-COOH MP的递送平台会能够将各不相同的滋生细胞加入适量各不相同的颗粒里变现疗法性生物工程原子核的目前尽情释放。EB杀菌工艺是一种种可不可以用于对承载滋生细胞的承载使用杀菌工艺的工艺，会为在监床软件应用中使用滋生细胞递送弄平高架道路。有关系选择：N3-PEG-SVAFA-PEG-N3N3-PEG-SSN3-PEG-SGPhosphoric acid-PEG-N3N3-PEG-PAA(1.8K)-DAN3-PEG-NHSN3-PEG-AlkeneACA-PEG-N3AC-PEG-N3AlKyne-PEG-N3COOH-PEG-N3MA-PEG-N3N3-PEG-EPON3-PEG-OH综上所述原创文章相关内容源于各种学术期刊或资料，要是有版权侵权请去咨询我们公司删去！