医学文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy小说作家：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie学术论文网页链接： 提要：A novel folate (FA) receptor-targeted superparamagnetic Fe3O4 nanoparticles (SPIONs) codelivering cisplatin (CDDP) and tissue factor pathway inhibitor-2 (TFPI-2) plasmid DNA (pDNA) was constructed. The core shell nanocomposites (FA-PEG-PEI@SPION-CDDP-TFPI-2) were composed of superparamagnetic Fe3O4 core that binds CDDP and TFPI-2 shell that combines with folate-polyethylene glycol-polyethyleneimine (FA-PEG-PEI) via electrostatic interaction. The shell containing FA-PEG-PEI and TFPI-2 plasmid was synthesized through amidation reaction and electrostatic adsorption and the core containing SPION-CDDP was modified by aldehyde sodium alginate. Proton nuclear magnetic resonance and Fourier transform infrared spectra on FA-PEG-PEI polymers showed characteristic peaks of various metabolites in corresponding frequency. Transmission electron microscopy image of FA-PEG-PEI@SPION-CDDP-TFPI-2 nanoparticles demonstrated a near-monodisperse spherical morphology, while dynamic light scattering studies indicated an intensity-average diameter of 149.5 nm. Zeta potential was 14.89 ± 1.83 mv and the final concentration of loaded CDDP was 100 ug/ml. Gel electrophoresis data showed that the nanocomposite would protect TFPI-2 pDNA from being digested by DNases. Compared with CNE-2 cells, the good targetability and better gene transfection efficiency (57.9%) were detected by Prussian blue iron stain and fluorescence analysis in HNE-1 cells in vitro. The results suggested the potential application of FA-PEG-PEI@SPION-CDDP-TFPI-2 as a multifunctional anticancer nanomedicine on targeting therapy for FR positive NPC.

构造 好几回种新型产品备孕叶酸（FA）肾上腺素受体靶向治疗的超顺带磁Fe3O4微米离子（SPION），其可一起递送顺铂（CDDP）和企业成分前提条件减缓剂-2（TFPI-2）质粒DNA（pDNA）。核壳纳米技术分手后复合的原材料(FA-PEG-PEI@SPION-CDDP-TFPI-2)由根据CDDP的超顺永磁铁Fe3O4核和经过静电能感应互相功效与孕妇叶酸聚乙二醇聚乙稀亚胺（FA-PEG-PEI）根据的TFPI-2壳构成的。经过酰胺化想法和静电能感应吸咐合成视频了带有FA-PEG-PEI和TFPI-2质粒的壳体，并且用水藻酸钠醛淡化了带有SPION-CDDP的体系化。FA-PEG-PEI水滑石物的质子核磁嗡嗡声和傅里叶变幻红外光谱图信息显示了某些频繁 下各方面分泌物的特点峰。散发出智能显微镜观察图案FA-PEG-PEI@SPION-CDDP-TFPI-2納米微粒表現出几乎单减少的球状姿态，而动态图片光散射研究分析反映出其強度最低值口径为149.5 nm.Zeta电势差为14.89±1.83 mv，负载电阻CDDP的后面溶液浓度为100 疑胶电泳的数据提示 ，納米结合材质都可以保护英文TFPI-2 pDNA因对DNA酶的代谢。与CNE-2細胞想必，普鲁士蓝铁染色剂和荧光分析一下在HNE-1細胞中检侧到更优质的靶向药物性和更优质的dna转染生产率（57.9%）。有关的个性化推荐：PLGA(20K)-PEG-OHPCL(5K)-PEG-OHPLA(2K)-PEG-OHOH-PEG-PLA(3K)6-NO2DA-PEG-OHOH-PEG-AAOH-PEG-SCMALKENE-PEG-OH CAS No.: 27274-31-3OH-PEG-PI(38K)PLGA(5K)-PEG-OHOH-PEG-AA-C30FA-PEG-OH这些论文项目的来源特殊杂志期刊或期刊论文，如果侵犯商标权请建立联系咱们移除！