资料:Gutenberg-style printing of self-assembled metal nanoparticle Aarays: electrostatic nanoparticle immobilisation and DNA-mediated transfer创作者： Zheng, Yuanhui论文资料连接：文献综述： HS–PEG–COOH was diluted in citrate solution while stirring at room temperature. An amount of DTT was added in the solution with molar ratio DTT/HS–PEG–COOH about 1/1. Here the DTT produces the free sulfhydryl bonds. The solution DTT–HS–PEG–COOH then was added into primary AuNPs solution while stirring at room temperature for about 15 min. The minimum amount of HS–PEG–COOH required to stabilize AuNPs was determined by the method similar to the BSA case. Two serial dilutions of the HS–PEG–COOH–AuNPs (Au@PEG) at pH=4 and pH=6.5 were prepared, varying the quantities of HS–PEG–COOH from 0 to 150 μg in 1 ml of AuNPs solution (μg  ml −1), and followed by UV-VIS spectrophotometric analysis. The minimum amount of HS–PEG–COOH necessary to coat AuNPs was deduced graphically from the concentration with which the absorbance at pH=4 becomes nearly constant. All of these solutions were measured by UV-Vis spectrophotometry. The size and shape of AuNPs were characterized by a transmission electron microscope (TEM).

在空调温度下打料的的同时，将HS-PEG-COOH直接稀释就可以在柠檬汁酸盐溶剂中。在溶剂内加如一定的量的DTT，DTT/HS–PEG–COOH的摩尔比约为1/1。于你来，DTT行成氧化钙含量巯基键。但是将DTT-HS-PEG-COOH溶剂加如到中级考试AuNP溶剂中，的同时在空调温度下打料约1分之五钟 min。不稳AuNP所需要的HS-PEG-COOH的不大量是确认近似于BSA的步骤设定的。HS-PEG-COOH-AuNPs的五种系稀释溶解液(Au@PEG)在pH=4和pH=6.5的水平下，将HS-PEG-COOH的量从0变迁到150 μg/1 ml金奈米级粒子剂液体（μg  ml-1），接下来使用UV-VIS分光光度实施分析。选择pH=4时吸光度基本上不变的有机废气浓度，以多边形途径推论出涂覆AuNP需提交的较大HS-PEG-COOH量。整个他们液体都实现红外光谱-常见分光光度法使用了測量。实现散发出电子无线高倍显微镜（TEM）对金奈米级粒子剂的长宽高和外形使用了定量分析。各种相关推存：CLS-PEG-SilaneCLS-PEG-FITCCLS-PEG-BiotinCLS-PEG-MalCLS-PEG-FACLS-PEG-SHCLS-PEG-NH2CLS-PEG-COOHCLS-PEG-SCmPEG-CLS8-ArmPEG-CLS4-ARMPEG-CLSCLS-PEG-CLS不低于文章标题內容种类以及杂志或医学文献，烦请抄袭请电话联系大家去除！