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SH-PEG-COOH介导的金纳米棒多功能表面修饰及细胞穿透肽偶联
发布时间:2025-07-23     作者:zyl   分享到:
论文参考文献:Surface Functionalized Gold Nanorods: Tracking and Observing Live Cell via Three Optical Signals写作者:Qiu, Xiaozhong; Wang, Yaling; Cui, Yanyan; Sun, Zhipeng; Liu, Ru; Huang, Wenhua; Zhao, Feng; Zu, Yan; Yuan, Hui; Chang, Xueling; Gao, and Xueyun论文参考文献环节://www.ingentaconnect.com/contentone/asp/jnn/2012/00000012/00000009/art00004内容提要:Surface functionalized gold nanorods (GNRs) with three optical signals and the ability to track and observe live cells was investigated. The Cetyltrimethyl Ammonium Bromide (CTAB) was used as the shape-directing agent to develop GNRs with uniform size and aspect ratio. The as-synthesized GNRs can strongly enhance the Raman signal of the attached MGITC on the GNRs. Polyethylene glycol (SH-PEG-COOH) and SH-PEG-FITC were attached with GNRs through ligand exchange with CTAB. They can stabilize the GNRs, reduce the cytotoxicity and provide fluorescence signal. A cell-penetrating peptide was covalently conjugated with SH-PEG-COOH to assist the GNRs to enter living cell. After cultured with the MCF-7 cells, the surfaced functionalized Au nanorods can enter the MCF-7 cells and be tracking with enhanced MGITC Raman signal, FITC fluorescence, and Rayleigh scatter signal.

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学习了具三种类型光讯号的面用途化金nm棒(GNR)或跟进和观察动物活生殖细胞的能力素质。采用16烷基三级甲等医院基溴化铵(CTAB)用于的形状导向性剂,规划设计出尺寸和纵行比平滑的GNR。组成的GNR就能够过强资料GNR上黏附的MGITC的拉曼讯号。聚乙二醇(SH-PEG-COOH)和SH-PEG-FITC使用与CTAB的配体交流与GNR连入。患者能够 稳固GNR,消减人体神经人体组织渗透性并就可以提供荧光信息。人体神经人体组织击穿肽与SH-PEG-COOH共价配合,以作用GNR进去活人体神经人体组织。与MCF-7人体神经人体组织一并陪养后,单单从表面官能化的Au纳米技术棒能够 进去MCF-7人体神经人体组织,接成提升的MGITC拉曼信息、FITC荧光和瑞利散射信息做出追综。有关系建议:Palmitic acid-PEG-MalPalmitic acid-PEG-NH2Palmitic acid-PEG-SCRB-PEG-DSPESTA-PEG-CHOSTA-PEG-COOHSTA-PEG-FASTA-PEG-FITCSTA-PEG-MalSTA-PEG-NBDSTA-PEG-NH2这些的文章项目来源地各期刊论文或期刊论文,比如图片侵权请去在线留言的全部删除!