DSPE-PEG-DBCO介导的脂质躯干面的功能化与海洋生物正构体合成视频联接：//www.researchgate.net/profile/Xiangdong-Xue-2/publication/390580878_Blocked_bioorthogonal_chemistry_enabled_switchable_bioorthosome_to_improve_liposomal_drug_delivery_for_glioblastoma_therapy/links/67f5bfb8e8041142a16fb0dc/Blocked-bioorthogonal-chemistry-enabled-switchable-bioorthosome-to-improve-liposomal-drug-delivery-for-glioblastoma-therapy.pdf我：Chao Wang, Jie Wu, Zhiran Duan, Yuqing Pan, Haijing Qu, Wei Cheng, Ning Wang, Han Chen, Xiaoli Gao,Mengqing Hou, Ying Zhang, and Xiangdong Xue节选：生物体正构体的光催化原理实现pet薄膜水合技巧制法了动物正构体。到底情况来讲一，15毫克豆类的融合物磷脂酰胆碱（PC）、5毫克低密度胆固醇、0.6毫克DSPE-PEG2000，0.3mg DSPE-PEG-PBA和0.1mg DSPE-PEG-DBCO的融合物是溶于在甲醇/氯仿悬浊液（1:3，v/v）中圆底烧瓶。食用旋轉式挥发器挥发萃取剂挥发器在烧瓶内外观行成薄脂质膜，陆陆续续实现真空环境晾干，以抓好完完全全除去剩余的萃取剂。得到的脂质膜用4mL水复水在多普勒彩超波解决下，将有2mg TMZ的PBS溶于。该之后将再水合的透明桌面液在11次食用Avanti Mini一挤机的聚氨酯膜（200 nm）以赢得不匀的脂质体。自此，MA（20毫克，0.06毫摩尔）将其申请加入脂质体悬浊液中，陆陆续续在摇床里孵育包夜以力促海洋动物学想法这成型pH的反应性硼酸酯键（PBA-MA）的行成，之后成型之后动物正构体的获得。比脂质体实现同的方式实现获得，到底情况来讲一具有不包括pH值的DBCO-MA脂质想法性和动物正交海洋动物学关闭，与DBCO-PBA脂质体，其上缺少常见葡萄品种糖能力外观。在休外调查中包封罗丹明B（RhB），而吲哚青绿色（ICG）则应用于肚子里所以脂质体组的动物遍布分折。译文翻译：Preparation of the BioorthosomeThe Bioorthosome was prepared using the thin-film hydrationtechnique. Specifically, a mixture of 15 mg soyphosphatidylcholines (PC), 5 mg cholesterol, 0.6 mg DSPE-PEG2000,0.3 mg DSPE-PEG-PBA, and 0.1 mg DSPE-PEG-DBCO wasdissolved in a methanol/chloroform solution (1:3, v/v) within around-bottom flask. The solvent was evaporated using a rotaryevaporator to form a thin lipid film on the flask's inner surface,followed by vacuum desiccation to ensure complete removal ofresidual solvents. The resulting lipid film was rehydrated with 4 mLof PBS containing 2 mg TMZ under ultrasonication. Therehydrated suspension was then passed 11 times through apolycarbonate membrane (200 nm) using an Avanti Mini-Extruderto obtain uniform liposomes. Thereafter, MA (20 mg, 0.06 mmol)was added to the liposome solution, which was subsequentlyincubated on a shaker overnight to promote the chemical reactionbetween PBA and MA. This resulted in the formation of pHresponsive borate ester bonds (PBA-MA), culminating in thesynthesis of the final Bioorthosome. Control liposomes weresynthesized employing the same methodology, specificallyincluding the DBCO-MA Lipo, which is devoid of pHresponsiveness and bioorthogonal chemistry shielding, as well asthe DBCO-PBA Lipo, which lacks glucose functionality on itssurface. For in vitro studies, rhodamine B (RhB) was encapsulated,while indocyanine green (ICG) was incorporated for in vivobiodistribution analysis across all liposome groups.

深圳杏彩体育平台 生物体提高相关的设备：DSPE-PEG-acidDSPE-PEG4-acidDSPE-PEG-AldDSPE-PEG-Amine，1069-79-0DSPE-PEG-azideDSPE-PEG5-azideDSPE-PEG-Biotin超过文章项目项目起源常见杂志期刊或医学文献，如果有侵犯肖像权请找公司卸载！