DSPE-PEG-NHS介导的CpG递送软件系统装修设计与抗体活性酶类品价链接代码：//link.springer.com/article/10.1007/s12257-020-0366-1小说作家：Jiwon Yang, Eun Seo Choi, Gayeon You & Hyejung Mok 小结：本着CpG寡脱氧核苷酸（CpG）还具有专业的纯天然免疫检测检测影响用，定制开拓CpG膜蛋白是推动提极有效率能率肿瘤纯天然免疫检测检测*的先决基本条件。本科研将1,2-二硬脂酰-sn-甘油-3-磷酸甲醇胺-N-[羟基虎珀酰亚胺（聚乙二醇）] (DSPE-PEG-NHS) 与聚氯乙烯亚胺 (PEI) 偶联，定制开拓出某种PEI-PEG-DSPE偶联物，可以作为营生物相融性的提极有效率能率CpG膜蛋白。企业定制开拓了四种PEIPEG-DSPE偶联物，多种不相同偶联物的PEI氧分子量不相同，DSPEPEG的表达数量只要相同，均展示出更为明显较低的癌内部系致癌性。基本一般说来，与经过纯天然PEI递送CpG相对比，以摩尔比0.1递送PEI (25 kDa)-PEG-DSPE和DSPE-PEG-NHS/（PEI的胺基）可造成RAW264.7癌内部系对CpG的吸收的效应最高，这机会是随着产生疏水性树脂脂质组成部分。除此以外，PEI-PEG-DSPE/CpG黏结物可引诱RAW264.7癌内部系更为明显产生癌内部系因素（TNF-α），其用与PEI/CpG黏结物等于。由此，PEI-PEG-DSPE偶联物可以作为营生物相融性的提极有效率能率膜蛋白，将纯天然免疫检测检测影响剂CpG递送货到巨噬癌内部系。古诗网：Considering the potent immune stimulation by CpG oligodeoxynucleotides (CpGs), the development of CpG carriers is a prerequisite for efficient cancer immunotherapy. In this study, we conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[hydroxyl succinimidyl (polyethylene glycol)] (DSPE-PEG-NHS) with polyethylenimine (PEI) to develop a PEI-PEG-DSPE conjugate that can serve as a biocompatible and efficient CpG carrier. Five types of PEIPEG-DSPE conjugates were developed, each with different molecular weights of PEI and different degrees of DSPEPEG modification, and all exhibited significantly lower cytotoxicity. In particular, compared to CpG delivery via natural PEI, delivery with PEI (25 kDa)-PEG-DSPE and DSPE-PEG-NHS/(amine groups of PEI) at a molar ratio of 0.1 resulted in a higher uptake of CpGs into RAW264.7 cells, probably because of the presence of a hydrophobic lipid moiety. In addition, PEI-PEG-DSPE/CpG complexes triggered significant cytokine secretion (TNF-α) from RAW264.7 cells, comparable to that triggered by PEI/CpG complexes. Thus, PEI-PEG-DSPE conjugates could serve as biocompatible and efficient carriers of the immune stimulator CpG to the macrophages.

山东杏彩体育平台 生物制品提供了涉及到服务：DSPE-PEG-VIP(二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-*血官催化活性肠肽)DSPE-PEG-GE11(二硬脂酰基磷脂酰甲醇胺-聚乙二醇-*内部上皮组织生長细胞因子EGFR靶向药物肽)DSPE-PEG-PTP(二硬脂酰基磷脂酰酒精胺-聚乙二醇-胰腺癌靶点肽)DSPE-PEG-FSHB(二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-暖巢癌靶向治疗肽)DSPE-PEG-R6H4（二硬脂酰基磷脂酰甲醇胺-聚乙二醇-PH相应性細胞穿膜肽)DSPE-PEG-SEARYL-R8（二硬脂酰基磷脂酰酒精胺-聚乙二醇-PH为了响应性组织穿膜肽)DSPE-PEG-SEARYL-PF6（二硬脂酰基磷脂酰酒精胺-聚乙二醇-PH响应的性人体细胞穿膜肽)DSPE-PEG-TH（二硬脂酰基磷脂酰无水乙醇胺-聚乙二醇-PH反映性细胞核穿膜肽)DSPE-PEG-OTC（二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-植物生长抑索肾上腺素受体靶向疗法肽)左右一篇文章相关内容主要来源多种刊物或资料，烦请版权侵权请认识.我删去！