文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

The FA-PEG-PEI@SPION-CDDP was constructed with SPION-CDDP (core) and FA-PEG-PEI (shell). Numerous detached electropositive amino groups in the shell covered SPION-CDDP nanoparticles were able to electrostatically adsorb electronegative TFPI-2 pDNA. To explore the binding ability of FA-PEG-PEI@SPION-CDDP with TFPI-2 pDNA, gel electrophoresis was observed in Figure 3(a). It was found that with the increasing mass ratio of FA-PEG-PEI@SPION-CDDP/TFPI-2 {Figure 3(a)~B (1 : 4) → C (1 : 2) → D (1 : 1)}, more TFPI-2 was blocked in sample well. When the mass ratio was equal to or more than 2 (Figure 3(a)~E), TFPI-2 pDNA was restricted completely in the well, indicating entirely adsorption and encapsulation by FA-PEG-PEI@SPION-CDDP. As a result, the relative mass ratio was determined as 2 : 1 to synthesize the final complex. To investigate the protection of TFPI-2-loaded complex (FA-PEG-PEI@SPION-CDDP-TFPI-2) against DNase-I degrading, FA-PEG-PEI@SPION-CDDP was mixed with overdose TFPI-2, then various content of DNase-I was added to run electrophoresis. As shown in Figure 3(b), compared with TFPI-2 migration without DNase-I in Figure 3(a)~B, the more DNase-I was added, the more detached TFPI-2 was degraded with more fade band of TFPI-2 migration. When DNase-I was equal to or higher than 15 units, the detached TFPI-2 (Figure 3(b)~J, K) was entirely digested except for the sample well. This evidence shed light on the enzymatic hydrolysis of DNase-I in TFPI-2 pDNA and indicated that FA-PEG-PEI@SPION-CDDP is feasible in adsorbing TFPI-2 and protecting pDNA from degradation. Thus pDNA could avoid digestion by DNase in media or blood before reaching the targeted cells, leading to an efficient transfection in tumors.

这位FA-PEG-PEI@SPION-CDDP用SPION-CDDP（管理的本质）和FA-PEG-PEI（设备壳）在校园营销推广活动的环节之中所构建。壳遍及的SPION-CDDP微米粒状中的多个分离处理的正电氨基还可以如何消除静电树脂吸附电负性TFPI-2 pDNA。探索性其综合能力素质FA-PEG-PEI@SPION-CDDP关于TFPI-2 pDNA，凝胶的作用电泳。得知现在产品品质比的增大FA-PEG-PEI@SPION-CDDP/TFPI-2{图3（a）~B（1:4）→C（1:2）→D（1:1）}，更高的TFPI-2在样板孔中被阻隔。当产品品质比等同于或超出2时，TFPI-2 pDNA完成规定在孔中，体现了完成被活性炭吸附和包封FA-PEG-PEI@SPION-CDDP.然后结果，相对来说产品品质比被确定好为2 : 1 生成然后的包覆物。TFPI-2环境下挽回物的保养功效探析(FA-PEG-PEI@SPION-CDDP-TFPI-2)抗DNase-I分解，FA-PEG-PEI@SPION-CDDP与过量饮用TFPI-2搭配，但是注入不一样纯度的DNase-I采取电泳。中都没有DNase-I的TFPI-2移迁优于，移除的DNase-I越小，TFPI-2的可降解层度越高，TFPI-1移迁的衰减带越小。当DNase-I=或优于11个企事业单位时，除备样孔外，提取的TFPI-2全被助消化。这个举证呈现了TFPI-2 pDNA中DNase-I的酶油脂水解，并揭示FA-PEG-PEI@SPION-CDDP在离心分离TFPI-2和维护pDNA受到化学降解工作方面是必须的。往往，pDNA需要避免出现在直达靶人体细胞前面被塑造基或血浆中的DNase肠蠕动，最后在癌症中进行有效率转染。涉及到引荐：OH-PEG-SSOH-PEG-SGLA-PEG-OHN-(3-hydroxypropyl) phthalimide-PEG-OHBenzyl-PEG-OHPLGA(20K)-PEG-OHPCL(5K)-PEG-OHPLA(2K)-PEG-OHOH-PEG-PLA(3K)6-NO2DA-PEG-OHOH-PEG-AAOH-PEG-SCM综上所述新闻稿件项目源于四种杂志或期刊论文，假如有侵犯著作权请连系我们大家清空！