文献资料：Tumor angiogenesis targeting and imaging using gold nanoparticle probe with directly conjugated cyclic NGR†笔者：Minghao Wu‡ ORCID logoa, Yanyan Zhang‡a, Ying Zhanga, Mingjie Wub, Menglin Wua, Hongyi Wua, Lin Caoa, Liang Lia, Xue Li*a and Xuening Zhang*a论文参考文献微信链接：//pubs.rsc.org/en/content/articlehtml/2018/ra/c7ra10155d前言：Preparation of FITC coupled GNPs–PEG@cNGR and GNPs–PEG.To prepare FITC coupled GNPs–PEG@cNGR and GNPs–PEG, 1 mg of the GNPs–PEG@cNGR probe, previously synthesized, was dispersed into 1 mL crosslinking reaction solution containing 7.56 mg Na2CO3, 1.06 mg NaHCO3, and 7.36 mg NaCl, sonicated for 2 min, and stored at 4 °C for 10 min. Subsequently, 0.3 mg FITC in 300 μL of DMSO was poured into the above solution under gentle and continuous stirring and incubated in the dark for 8 h at 4 °C. Next, 20 μL NH4Cl solution (5 M) was poured into the above solution under gentle and continuous stirring to terminate the above reaction and then incubated in the dark for 2 h at 4 °C. The final GNPs–PEG@cNGR@FITC was retrieved by ultrafiltration with a Millipore centrifugal filter unit (Amicon Ultra-0.5; MWCO 100[thin space (1/6-em)]000), washed three times, and resuspended in 0.5 mL of PBS (pH 7.4).In addition, FITC-conjugated GNPs–PEG (GNPs–PEG@FITC) was also prepared as a control. Briefly, the preparation was similar to the fabrication of GNPs–PEG@cNGR, but 220 μL SH–cNGR (1 mg mL−1) was replaced by 15 μL 2-aminoethanethiol (20 mM).

準備FITC解耦的GNP——PEG@cNGR还有GNP-PEG，1毫克GNP-PEGPEG@cNGR将的时候转化成的检测器离心分离到包含有7.56mg Na2CO3、1.06mg NaHCO3和7.36mg NaCl的1mL化学交联反响悬浊液中，高周波处里215分钟的时间，在4°C下保存1015分钟的时间。随着，在清新持续打料下，将0.3mg FITC不能溶解300μL DMSO中倒这些水氢氧化钠稀硫酸中，在4℃下遮光孵育8个钟头。下面来，在清新持续打料下将20μL NH4Cl水氢氧化钠稀硫酸（5M）倒这些水氢氧化钠稀硫酸中以撤消这些作用，然后呢在4°C传到黑暗地里孵育2个钟头。终究的GNPPEG@cNGR@便用Millipore离心法全自动过滤器中器第一单元（Amicon Ultra-0.5；MWCO 100[薄发展空间（1/6-em）]000）采取反渗透装置，回报FITC，干洗俩次，并举新悬停在0.5 mL PBS（pH 7.4）中。与此同时，FITC偶联的GNP-PEG（GNP-PEG）PEG@FITC)也提供做为对应。简如何理解之，制法时候内似于GNP的开发——PEG@cNGR，但220μL SH-cNGR（1 mg mL-1）被15μL 2-氨基乙硫醇（20 mM）所被淘汰。有关个性化推荐：Biotin-PEG-PFPDiethoxylsilane-PEG-BiotinMonoethoxylsilane-PEG-BiotinBiotin-PEG-TretinoinNTA-PEG-BiotinBiotin-PEG-SSBiotin-PEG-SGBiotin-PEG-HZBiotin-PEG-SVABiotin-PEG-ACBiotin-PEG-CHODBCO-PEG-BiotinBiotin-PEG-FA上面的稿件游戏内容种类分类期刊杂志或资料，要是有商标侵权请找话题公司移除！