学术论文：Evaluation of the physicochemical properties and the biocompatibility of polyethylene glycol-conjugated gold nanoparticles: A formulation strategy for siRNA delivery诗人：Kamil Rahme a b c 1, Jianfeng Guo d 1, Justin D. Holmes a b, Caitriona M. O’Driscoll资料联结：英文论文：Recently, the potential of gold nanoparticles (AuNPs) for transporting drugs, proteins and genetic materials has been demonstrated. Previously, our laboratory synthesised positively charged, surfactant-free AuNPs in water by the reduction of gold (III) chloride (AuCl3) using hydroxylamine hydrochloride (NH2OH·HCl) in the presence of l-cysteine methyl ester hydrochloride (HSCH2CH(NH2)COOCH3·HCl) as a capping agent. These AuNPs, which achieve higher cell viability in comparison to cetyl trimethyl ammonium bromide (CTAB, a surfactant)-capped counterparts, have demonstrated potential for siRNA delivery. However, it is well known that systemic administration of cationic delivery systems without biological stablising moieties causes non-specific binding with negatively charged serum proteins, resulting in particle aggregation and opsonisation. Consequently, highly stable AuNPs capped with l-cysteine methyl ester hydrochloride conjugated to poly(ethylene glycol) (PEG) were synthesised in this study. PEGylation enhanced the biocompatibility of the AuNPs by reducing toxicity in a range of cell types, by inhibiting interaction with serum proteins thus avoiding aggregation, and, by providing protection against degradation by nucleases. Moreover, these PEGylated AuNPs formed nanoparticles (NPs) with siRNA (which was first compacted with protamine), and had a diameter within the nanoscale range (∼250 nm) and a near neutral surface charge (∼10 mV). In the future a bifunctional PEG chain on the AuNPs (i.e., SH-PEG-NH2, SH-PEG-COOH) will be used to facilitate conjugation of a targeting ligand to enhance cell specific uptake.

金納米颗粒（AuNP）在运输业治疗药物、血清质和遗传基因材质个方面的价值到了得知。前，企业的实验报告室在l-半胱氨酸甲酯硫酸盐（HSCH2CH（NH2）COOCH3·HCl）算作封端剂的留存下，的使用硫酸羟胺（NH2OH·HCl）备份氯化金（III）（AuCl3），在水底分解带正带电粒子、无表面层抗逆性剂的AuNP。与16烷基三甲医院基溴化铵（CTAB，另外一种外壁吸附性剂）封的对应着物相对来说，等AuNP具备挺高的细胞膜杀灭率，都已经 证实了siRNA递送的有潜力。但，不能动物可靠组成部分的阳化合物递送系统的的身上给药会造成的与带负正电荷的血清核蛋白的非特喜欢的人运用，故而造成的颗粒肥料集聚和调整。以至于，本研究探讨制成了用与聚乙二醇（PEG）共轭的l-半胱氨酸甲酯酸洗盐封web端极度可靠的AuNP。聚乙二醇化能够大幅度降低一一种组织细胞类别的致毒、限制与血清蛋白质的上下级做用而使尽量不要密集，甚至给予以免核酸酶光降解的庇护，加强了AuNP的怪物混溶性。与此同时，这部分聚乙二醇化的AuNP与siRNA（一方面用鱼精核蛋白级配碎石）出现纳米技术级小粒（NP），厚度在纳米技术级级标准内（约250 nm），表层自由电荷介于比较适中（约10 mV）。中国未来，AuNP上的双特点PEG链（即SH-PEG-NH2、SH-PEG-COOH）将广泛用于推动靶向治疗配体的偶联，以提高细胞膜特女性朋友摄入。有关于比较适合：Biotin-PEG-FABiotin-PEG-NHSAlkyne-PEG-BiotinSilane-PEG-BiotinLA-PEG-BiotinIA-PEG-BiotinBiotin-PEG-ACAN3-PEG-BiotinOPSS-PEG-BiotinBiotin-PEG-MalBiotin-PEG-SCMSH-PEG-BiotinBiotin-PEG-OH以上相关内容软文相关内容源头特殊中文核心期刊或文献综述，告之商标侵权请找让我们删出！