论文：Endocytic Mechanisms of Graphene Oxide Nanosheets in Osteoblasts, Hepatocytes and Macrophages笔者：Javier Linares†M. Concepción Matesanz†Mercedes Vila‡§⊥M. José Feito†Gil Gonçalves⊥María Vallet-Reg퇧Paula A. A. P. Marques⊥M. Teresa Portolés文献资料联接： 文献综述：Nano-graphene oxide (GO) has attracted great interest in nanomedicine due to its own intrinsic properties and its possible biomedical applications such as drug delivery, tissue engineering and hyperthermia cancer therapy. However, the toxicity of GO nanosheets is not yet well-known and it is necessary to understand its entry mechanisms into mammalian cells in order to avoid cell damage and human toxicity. In the present study, the cellular uptake of pegylated GO nanosheets of ca. 100 nm labeled with fluorescein isothiocyanate (FITC-PEG-GOs) has been evaluated in the presence of eight inhibitors (colchicine, wortmannin, amiloride, cytochalasin B, cytochalasin D, genistein, phenylarsine oxide and chlorpromazine) that specifically affect different endocytosis mechanisms. Three cell types were chosen for this study: human Saos-2 osteoblasts, human HepG2 hepatocytes and murine RAW-264.7 macrophages. The results show that different mechanisms take part in FITC-PEG-GOs uptake, depending on the characteristics of each cell type. However, macropinocytosis seems to be a general internalization process in the three cell lines analyzed. Besides macropinocytosis, FITC-PEG-GOs can enter through pathways dependent on microtubules in Saos-2 osteoblasts, and through clathrin-dependent mechanisms in HepG2 hepatocytes and RAW-264.7 macrophages. HepG2 cells can also phagocytize FITC-PEG-GOs. These findings help to understand the interactions at the interface of GO nanosheets and mammalian cells and must be considered in further studies focused on their use for biomedical applications.

微米氧化物苯（GO）是由于其一直有的化学性质还有在类药传送、安排水利工程和热疗等食草动物药学域的因素用途，引发的了患者对微米药学的有效爱好。可是，GO微米片的渗透性尚不了解，非常有必要条件了解其进去母乳喂奶食草动物组织的缘由，以杜绝组织挤压伤和我们身体渗透性。在本探讨中，在七种可以抑药品（雨暗仙素、渥曼宁、阿米洛利、生殖肿瘤受损细胞放松下来素B、生殖肿瘤受损细胞放松下来淀粉酶D、有机染料木素、苯胂氧化反应物和氯丙嗪）的会出现下，评估报告格式了用异硫氰酸荧光素（FITC-PEG-GO）标示的约100nm的聚乙二醇化GO微米片的生殖肿瘤受损细胞摄食，这样可以抑药品好一点反应不一的内吞策略。本设计抉择了两类组织癌癌人体细胞分类：人Saos-2成骨组织癌癌人体细胞、人HepG2肝组织癌癌人体细胞和小鼠RAW-264.7巨噬组织癌癌人体细胞。可是呈现，与众不同多种与众不同血受损生殖受损人体神经内部类的特点，与众不同的考核机制化加入了FITC-PEG-GOs的摄入。虽然，在所进行分析的八种血受损生殖受损人体神经内部系中，大粒子血受损生殖受损人体神经内部不断增加尽管不是个都的内化过程中 。出了巨噬血受损生殖受损人体神经内部效应外，FITC-PEG-GOs还应该确认Saos-2成骨血受损生殖受损人体神经内部中忽略性微管的条件进到，并确认HepG2肝血受损生殖受损人体神经内部和RAW-264.7巨噬血受损生殖受损人体神经内部中的网格蛋清忽略性考核机制化进到。HepG2体癌细胞也就能够摧毁FITC-PEG-GOs。这出现 不利于掌握GO纳米级片和辅乳期動物体癌细胞软件界面上的间接功用，在进步骤设计其在动物医美运用中的运用时就必须加上选择。相应网友推荐：FITC-PEG-DMGFITC-PEG-FAFITC-PEG-BiotinOH-PEG-FAMNH2-PEG-FAMNTA-PEG-FITCFAM-PEG-N3FITC-PEG-ACFITC-PEG-ACAFITC-PEG-AlkyneFITC-PEG-AzithromycinFITC-PEG-CHOFITC-PEG-DTPAFITC-PEG-Estrogen左右稿件信息内容从何而来各样学术期刊或专著，烦请侵权行为请关联他们删去！