文章：血清火大肠杆菌培养有效降低 ApoE 介导的 D-Lin-MC3-DMA 脂质纳米级粉末的摄取量下载链接：//www.beilstein-journals.org/bjnano/articles/16/57我们：德米安·范斯特拉滕， 卢克·范德·谢波普， 罗文·弗朗特， 彼得·维德和 雷蒙德·M·希弗勒斯节选：抽象性的奈米级级级颗粒束在类药物递送探究中更好地发挥着至关为重点的的角色。奈米级级级颗粒束给药后在其外壁生成的淀粉酶质冠因而对其效能的特殊影向而大受重视。脂质奈米级级级颗粒束 (LNP) 依赖性淀粉酶质冠的生成来体现其靶向治疗性。以上淀粉酶质-奈米级级级颗粒束上下级的角色常常情况下*初在使用离体组织癌細胞系仿真模型使用探究，重点途径*终熟悉 LNP 在身体内的生物制品分布图和载药递送使用率。在离体组织癌細胞系陪养中，常常情况下会在陪养基中调用胎牛血清 (FCS) 以能提供营养摄入并提高网站组织癌細胞系青春活力和生长发育。常常情况下对 FCS 使用热消灭当心止补保障体系统刺激启动。其实，该环节对淀粉酶质冠生成和以致对 LNP 的交叉性的影向尚不明白。本诗，咱们探究了血清热去火消灭对有效 D-lin-MC3-DMA (MC3) 或 C12-200 (C12) 可电离脂质的 LNP 中淀粉酶质冠生成的影向。在有效未治理或热消灭血清的陪养基中，法测定了LNP的组织癌細胞系摄食量和siRNA递送使用率。从机能上讲，咱们出现 载脂淀粉酶E（一个对MC3 LNP取向性至关为重点的淀粉酶冠营养成分）在FCS热消灭后保持稳定可靠性处理和的交叉性性减小，以此对MC3 LNP的摄食量和物料递送带来负面信息影向，但对C12 LNP则无影向。咱们的探究成果着重指出了离体试验中被强毒的方面的为重点性，以上方面有机会会故意中影向LNP的效能。以上出现 能助加强离体探究淀粉酶冠生成的情况报告，并解决LNP激发中的差别。

AbstractNanoparticles play a crucial role in drug delivery research. The protein corona that develops on the surface of nanoparticles after administration has garnered substantial attention due to the significant effects it has on their performance. Lipid nanoparticles (LNPs) depend on protein corona formation to mediate their targeting. Such protein–nanoparticle interactions are often initially studied using in vitro cellular models aiming to eventually understand biodistribution and cargo delivery efficiency of the LNPs in vivo. For in vitro cell culture, fetal calf serum (FCS) is supplemented to culture media to provide nutrients and promote cell viability and growth. Heat inactivation of FCS is often performed to prevent complement system activation. However, the effect of this process on protein corona formation and, in turn, LNP functionality is unclear. Here, we investigated the effects of serum heat inactivation on protein corona formation on LNPs containing D-lin-MC3-DMA (MC3) or C12-200 (C12) ionizable lipids. Cellular uptake and siRNA delivery efficiency of the LNPs were determined in media containing untreated or heat-inactivated serum. Mechanistically, we found that apolipoprotein E, a protein corona component that is crucial for MC3 LNP tropism, displayed reduced stability and functionality upon heat inactivation of FCS, thereby negatively influencing uptake and cargo delivery of MC3 LNPs, but not C12 LNPs. Our results underline the importance of overlooked factors in in vitro experiments that can inadvertently affect LNP performance. These findings can help to improve protocols to study protein corona formation in vitro and prevent bias in LNP development.深圳杏彩体育平台 动物供给有关系商品：DPPE-PEG-COOHDSPE-PEG-Glutamic acidmPEG-DEPEICG-PEG-DLPEDSPE-MAL，DSPE-MaleimideDSPE-PEG-RVG29  二硬脂酰基磷脂酰酒精胺-聚乙二醇-狂犬病疫情肽DSPE-PEG2K-RVG29以下散文介绍来源地各样论文期刊或专著，以免抄袭请关系我门删除文件！