mPEG-DSPE/阿霉素胶束的制备及其淋巴结转移治疗效果研究
学术论文：MPEG-DSPE配位高分子化合物胶束使用于淋巴结肿大结转交的经淋巴结肿大放疗化疗链接转换：//www.sciencedirect.com/science/article/abs/pii/S0378517315002902作著：雪莉，董 青，严志强，维岳 路，冯莹莹，曹 协 阿，谢作旭，苏冰霞，刘 敏阿节选：本理论研究分离纯化了甲基聚乙二醇-二硬脂酰磷脂酰无水乙醇胺/阿霉素（MPEG-DSPE/DOX）胶束应用于*腮腺腺结传递。分离纯化的MPEG-DSPE/DOX胶束呈圆形，粒级为20 ± 5nm。A375内部对DOX和MPEG-DSPE/DOX胶束的摄取量率分別为51.2%和88.7%。RAW264.7内部对MPEG-DSPE/罗丹明B胶束的腐化率比单单选用罗丹明B低17.2倍。皮内接种后，MPEG-DSPE胶束经腮腺腺管降解，在腘窝腮腺腺结中积聚。 MPEG-DSPE/DOX胶束较单单DOX能突出消减接种脏器皮内进行的影响。制定A375内部腮腺腺结传递裸鼠模形，皮内接种后，MPEG-DSPE/DOX胶束组腘窝腮腺腺结和髂窝腮腺腺结总重量均突出高于生活生理盐水组和DOX组。MPEG-DSPE/DOX胶束能很好攻击腘窝腮腺腺结和髂窝腮腺腺结中的*内部。由此可见说明，MPEG-DSPE胶束都是种很有未来的*腮腺腺结传递的药递送设备。 AbstractLymph node metastasis is one of the major pathways for tumor formation and it is difficult to deliver chemotherapeutics at therapeutic concentrations to lymph node metastasis. This study prepared methyl poly(ethylene glycol)-distearoylphosphatidylethanolamine/doxorubicin (MPEG-DSPE/DOX) micelle for the treatment of lymph node metastasis. The MPEG-DSPE/DOX micelle prepared were of spherical morphology with a particle size of 20 ± 5 nm. The uptake rates of DOX and MPEG-DSPE/DOX micelle by A375 cells were 51.2% and 88.7%, respectively. The phagocytosis rate of MPEG-DSPE/Rhodamine B micelle by RAW264.7 cells was 17.2-fold lower than for Rhodamine B alone. After subcutaneous injection, MPEG-DSPE micelle underwent lymphatic absorption and accumulated in popliteal lymph nodes. MPEG-DSPE/DOX micelle significantly alleviated damage to the subcutaneous tissue of the injection sites compared with DOX alone. We established a model of nude mice bearing lymph node metastasis of A375 cells. After subcutaneous injection, the weights of both the popliteal and iliac lymph nodes of the MPEG-DSPE/DOX micelle group were significantly lower than in the saline and DOX groups. MPEG-DSPE/DOX micelle effectively killed the tumor cells in popliteal and iliac lymph nodes. In conclusion, MPEG-DSPE micelle is a promising drug delivery system for the treatment of lymph node metastasis.
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