文章：一起抑制 CD47 和 PD-L1 可强化先性情性和融入性胃癌免疫检测反响或者体细胞分子放出外链：//www.thelancet.com/article/S2352-3964(19)30158-6/fulltext作著：舒 莲，谢锐 志，叶玉英，谢晓东，李淑慧，路玉生，李碧飞，程云龙，弗拉基米尔·卡塔纳耶夫，李嘉节选：MAL-PEG-DOPE的分解成MAL-PEG-DOPE的制作而成基准以往公布的内容。适用EDC/NHS技术工艺将MAL-PEG-COOH的羧基与DOPE的胺基紧密搭配。基本做法内容如下：将30mg羧基掩盖的PEG溶解二氯二氧化氮中，并与5mgEDC和4mgNHS比调，常温下陆续搅拌器2h。之后参与8mg DOPE（MAL-PEG-COOH∶DOPE=1∶1，摩尔比），N2保护英文下发生不起作用迟钝一晚。将发生不起作用迟钝乙酰乙酸在自动旋转视频蒸发器掉仪中干热至大方面二氯二氧化氮，之后参与冷乙腈中。未发生不起作用迟钝的DOPE在2414g下抽滤10min，不溶解冷乙腈。上清液在自动旋转视频蒸发器掉仪中干热为稀脂质。将膜用DD润滑化。将发生不起作用迟钝乙酰乙酸倒入透析袋（分子式量= 8 k Da）中，并转换至50 mL DD水硫酸铜溶液中，常温下发生不起作用迟钝48每小时，分割分离的EDC/NHS/MAL-PEG-COOH。终究乙酰乙酸DOPE-PEG-MAL接着用冻干机冷冻冰箱。为验正DOPE-PEG-MAL的紧密搭配，对试样参与了核磁检查现象波谱解析。Synthesis of MAL-PEG-DOPESynthesis of MAL-PEG-DOPE refers to previously published articles [22,23]. The conjugation of carboxyl groups of MAL-PEG-COOH to the amine groups of DOPE was accomplished using the EDC/NHS technique. The process was carried out as follows: 30 mg carboxyl-modified PEG was dissolved in dichloromethane and mixed with EDC (5 mg) and NHS (4 mg). The solution was stirred continuously for 2 h at room temperature. Subsequently, 8 mg DOPE (MAL-PEG-COOH: DOPE =1:1, molar ratio) was added, and the reaction proceeded overnight under nitrogen. The reaction product was dried out most dichloromethane in rotary evaporator and then added to cold acetonitrile. The unreacted DOPE was centrifuged at 2414g for 10 min which was insoluble in cold acetonitrile. And the supernatant was dried to thin lipid in rotary evaporator. The film was hydrated with DD water. The reaction product was enclosed in dialysis bag (MW = 8 k Da) and transferred into 50 mL of DD water solution to separate free EDC/ NHS/ MAL-PEG-COOH at room temperature for 48 h. The final product DOPE-PEG-MAL was subsequently freezed by lyophilizer. To confirm the DOPE-PEG-MAL conjugation, the samples were examined by nuclear magnetic resonance spectroscopy.

广州杏彩体育平台 生物技术可以提供相关内容软件：DSPE-PEG-NBDDSPE-PEG-PDPDSPE-PEG-Anti-rat CC531mAb（二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-*靶向治疗淀粉酶)DSPE-OPSSDSPE-PEG-SCLyP-1-PEG-DSPECy7-DSPETAT-PEG-DSPE左右短文相关内容由来几大类期刊论文或医学文献，若有抄袭请连接我国刪除！