论文参考文献：此外阻绝 CD47 和 PD-L1 可增加天先性性和转变性胃癌免疫系统反馈及及癌细胞细胞施放联接：//www.thelancet.com/article/S2352-3964(19)30158-6/fulltext小编：舒 莲，谢锐 志，叶玉英，谢晓东，李淑慧，路玉生，李碧飞，程云龙，弗拉基米尔·卡塔纳耶夫，李嘉节选：MAL-PEG-DOPE的分解成MAL-PEG-DOPE的提炼关联性原先提出的的文章。通过EDC/NHS工艺将MAL-PEG-COOH的羧基与DOPE的胺基融入。具体的技巧如表：将30mg羧基淡化的PEG易溶二氯甲烷气体气体中，并与5mgEDC和4mgNHS混，恒温下不断打料2h。第三融入8mg DOPE（MAL-PEG-COOH∶DOPE=1∶1，摩尔比），惰性气体保养下不起作用過夜。将不起作用终终化合物在飞速转动化掉仪中粗糙至大一部分二氯甲烷气体气体，第三融入冷乙腈中。未不起作用的DOPE在2414g下离心分离处理10min，不易溶冷乙腈。上清液在飞速转动化掉仪中粗糙为稀脂质。将膜用DD润滑化。将不起作用终终化合物置入透析袋（氧分子量= 8 k Da）中，并迁移至50 mL DD水溶剂中，恒温下不起作用48每小时，分离处理自由的EDC/NHS/MAL-PEG-COOH。终结终终化合物DOPE-PEG-MAL后来用冻干机急冻。为了能够认证DOPE-PEG-MAL的融入，对印刷品展开了磁共振现象波谱了解。Synthesis of MAL-PEG-DOPESynthesis of MAL-PEG-DOPE refers to previously published articles [22,23]. The conjugation of carboxyl groups of MAL-PEG-COOH to the amine groups of DOPE was accomplished using the EDC/NHS technique. The process was carried out as follows: 30 mg carboxyl-modified PEG was dissolved in dichloromethane and mixed with EDC (5 mg) and NHS (4 mg). The solution was stirred continuously for 2 h at room temperature. Subsequently, 8 mg DOPE (MAL-PEG-COOH: DOPE =1:1, molar ratio) was added, and the reaction proceeded overnight under nitrogen. The reaction product was dried out most dichloromethane in rotary evaporator and then added to cold acetonitrile. The unreacted DOPE was centrifuged at 2414g for 10 min which was insoluble in cold acetonitrile. And the supernatant was dried to thin lipid in rotary evaporator. The film was hydrated with DD water. The reaction product was enclosed in dialysis bag (MW = 8 k Da) and transferred into 50 mL of DD water solution to separate free EDC/ NHS/ MAL-PEG-COOH at room temperature for 48 h. The final product DOPE-PEG-MAL was subsequently freezed by lyophilizer. To confirm the DOPE-PEG-MAL conjugation, the samples were examined by nuclear magnetic resonance spectroscopy.

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