参考文献：具备增加粘液融合性的奶外泌体可于口服药递送siRNA链接转换：//pubs.rsc.org/en/content/articlehtml/2020/bm/d0bm01497d笔者：Matthew R. Warren a、 Chenzhen 张 a、 Armin Vedadghavami a、 Krister Bokvist b、 Pradeep K. Dhal c和 Ambika G. Bajpayee * ad节选：荧光共轭 DSPE-PEG-叠氮化物的分离纯化。 在使用DSPE-PEG-叠氮化物（下例通称DPA）在 mExo 表皮行成疏水修改 PEG 金属涂层。先是将 DPA 以 2 mg mL −1的质量渗透压融化在 DMSO 中，进而逐滴修改到 1× PBS 中并不停的绞拌，使 DPA 的结果英文质量渗透压为 0.02 mg mL −1（7 μM），得以分离纯化水存量饱和水氢氧化钠溶液。这对所涉 DPA 质量渗透压批量的探究（即预估 mExo 表皮过载和锚保持稳定义探究），DPA完成叠氮化物-DBCO 打开电学表现与荧光要素 DBCO-Cy5 融合。将 DBCO-Cy5（DMSO 中质量渗透压为 1 mg mL −1）逐滴修改到水 DPA 存量液中，不停的绞拌，使结果英文摩尔比值 1 [细下划线（1/6-em）]：[细下划线（1/6-em）]4。进而在高温下悄悄的摆动该饱和水氢氧化钠溶液以加速打开表现打开表现后，将所含DPA-Cy5和过多会DBCO-Cy5的饱和水氢氧化钠溶液直接的修改到外泌体样品管理中去修改。古诗网：Preparation of fluorescent-conjugated DSPE-PEG-Azide. DSPE-PEG-Azide (hereby DPA) was utilized to enable a hydrophobically-inserted PEG coating on the mExo surface. An aqueous stock solution was prepared by first dissolving DPA in DMSO at 2 mg mL−1, followed by dropwise addition to 1× PBS with continuous stirring for a final DPA concentration of 0.02 mg mL−1 (7 μM). For studies involving quantification of DPA concentration (i.e. measurement of mExo surface loading and anchor stability studies), DPA was conjugated to the fluorescent moiety DBCO-Cy5 via the azide-DBCO click chemistry reaction. DBCO-Cy5 (1 mg mL−1 in DMSO) was added dropwise with continuous stirring to aqueous DPA stock for a 1[thin space (1/6-em)]:[thin space (1/6-em)]4 final molar ratio. This solution was then incubated for 6 h at room temperature with light shaking to facilitate the click reaction. After the click reaction, the solution containing DPA-Cy5 and excess DBCO-Cy5 was added directly to exosome samples for insertion.

兰州杏彩体育平台 生物技术展示关于成品：DSPE-PEG-Cy5DSPE-PEG-mAb 2C5（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*靶向疗法球蛋白)DSPE-PEG-OPSSCy5-DSPEDSPE-PEG-TetrazineDSPE-PEG-DAAsp6-PEG-DSPEDSPE-PEG2000-NGRXpeptide-PEG-DSPE之上软文游戏内容收入各种类型论文期刊或参考文献，见谅知识产权侵权请连系我国删去！