资料：融入共整合光学显微镜、dSTORM 和质谱技术应用呈现与纳米级架构脂质各种载体在血脑深层穿线操作过程中想关的淀粉酶质冠层的变迁联结：//pubs.rsc.org/en/content/articlehtml/2022/nr/d2nr00484d诗人：Matteo Battaglini ORCID ，Natalia Feiner bc， Christos Tapeinos a，Daniele De Pasquale a， Carlotta Pucci a，Attilio Marino a，Martina Bartolucci d，Andrea Petretto d，Lorenzo Albertazzi bc和 Gianni Ciofani 节选：物料和做法LMNV制得脂质磁铁nm质粒载体 (LMNV) 的制造技术服务协议拍成电影自机构班组以上的设计，结合实际了热多普勒彩超波和髙压均质化 (HPH) 的方法。20简在于之，机构将区别的脂质分层在一齐，比如 2.5 mg 油酸（Sigma-Aldrich）、25 mg 1-硬脂酰-rac -甘油（Sigma-Aldrich）、2.5 mg 油酸（Sigma-Aldrich）、2.5 mg 1,2-二棕榈酰-rac-甘油-3-磷酸胆碱（Sigma-Aldrich）和 4 mg 1,2-二硬脂酰-sn-甘油-3-磷酸无水工业乙醇胺与共轭甲氧基聚乙二醇 (mPEG-DSPE) (5000 Da, Nanocs)，同时 84.5 μl 超顺磁铁被氧化铁nm阿尔法粒子 (SPION) 的无水工业乙醇水溶液 (3 nm 尺寸，15 wt%；美设计nm村料机构) 装入 6 ml 玻离小瓶中。将3 ml打火（70 °C）的Tween® 80 (Sigma-Aldrich) 硫酸铜溶液 (1.0 wt%) 进入脂质/SPION消减体中，并安全应用超声波检查清洗波探测器 (Fisherbrand™ Q125 Sonicator) 实现超声波检查清洗治理14分种（波动30%，耗油率120 W）。超声波检查清洗治理后，安全应用均质机以100 [细单引号（1/6-em）]000 psi的压强对相溶物实现低压变压器均质治理（共实现5次低压变压器均质治理）。奈米各种承载在4 °C时以16 [细单引号（1/6-em）]000 g抽滤90分种（共实现3次）实现纯化，接下来重消减于水面。只为实现共对焦激光散斑，LMNV 安全应用荧光 Vybrant DiO 体细胞标签活性染料（Invitrogen）实现标签。将 5 mg 奈米各种承载与 20 μM DiO 在 37 °C 下孵育 2 个钟头，接下来在 4 °C 时以 16 [细单引号（1/6-em）]000 g抽滤 90 分种（第三次）实现清洗。相对会直接js随机数光学材料新建高倍显微镜 (dSTORM) 讲解，在制法操作过程开国少将 3 mg mPEG-DSPE（5000 Da，Nanocs）与 1 mg DSPE-PEG-Cy3（5000 Da）相溶实现脱色。Materials and methodsLMNV preparationThe protocol for the fabrication of lipid magnetic nanovectors (LMNVs) was adapted from previous works of our group combining hot ultra-sonication and high-pressure homogenization (HPH) methods.20 Briefly, we mixed different lipids including 2.5 mg of oleic acid (Sigma-Aldrich), 25 mg of 1-stearoyl-rac-glycerol (Sigma-Aldrich), 2.5 mg of oleic acid (Sigma-Aldrich), 2.5 mg of 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (Sigma-Aldrich), and 4 mg of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine with conjugated methoxyl poly(ethylene glycol) (mPEG-DSPE) (5000 Da, Nanocs) with 84.5 μl of an ethanol solution of superparamagnetic iron oxide nanoparticles (SPIONs) (3 nm diameter, 15 wt%; US Research Nanomaterials Inc.) into a 6 ml glass vial. 3 ml of pre-warmed (70 °C) Tween® 80 (Sigma-Aldrich) solution (1.0 wt%) were added to the lipid/SPION dispersion and sonicated using an ultrasonic tip (Fisherbrand™ Q125 Sonicator) for 15 min (amplitude 30%, 120 W). After the sonication, the mixture underwent high-pressure homogenization with a homogenizer at 100[thin space (1/6-em)]000 psi (5 passages of high-pressure homogenization were performed). The nanovectors were purified by centrifugation at 16[thin space (1/6-em)]000g for 90 min at 4 °C (three passages) and then re-dispersed in water. For confocal imaging, LMNVs were labeled with the fluorescent Vybrant DiO cell-labeling dye (Invitrogen) by incubating 5 mg of nanovectors with 20 μM of DiO for 2 h at 37 °C and then washing them by centrifugation at 16[thin space (1/6-em)]000g for 90 min at 4 °C (three passages). For Direct STochastic Optical Reconstruction Microscopy (dSTORM) analysis, the staining was obtained by mixing 3 mg of mPEG-DSPE (5000 Da, Nanocs) with 1 mg of DSPE-PEG-Cy3 (5000 Da) during the preparation procedure.

山东杏彩体育平台 生物体提供了重要性服务：M2pep-PEG-DSPEEB1-PEG-DSPECPP-PEG-DSPECCK8-PEG-DSPEFSHB(QCHCGKCDSDSTDCT)-PEG-DSPEGRGDS-PEG-DSPEMMPs-PEG-DSPEWSW(WSWGPYS)-PEG-DSPELyP-1-PEG-DSPEVIP-PEG-DSPECREKA-PEG-DSPEAsp8-PEG-DSPE这些新闻稿件信息内容的来源常见学术期刊或论文，如果侵犯肖像权请联系起来我们的全部删除！