文献：PLGA-卵磷脂-PEG核壳纳米粒子用于癌症靶向*

链接：http://www.worldscientific.com/doi/abs/10.1142/S1793984411000359

作者：艳丽， 里克·奥克塔维安蒂·托伊普， 陶鹏， 和 林诗琳

摘要：

我们报道了一种多功能聚乳酸-乙醇酸共聚物 (PLGA)-卵磷脂-聚乙二醇 (PEG) 核壳纳米粒子 (NPs)，该纳米粒子兼具脂质体和聚合物纳米粒子的优点，可用于递送化疗药物。该纳米粒子的粒径、表面电荷和表面官能团可通过各种配方参数轻松调节，且重复性高，例如脂质/聚合物、1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺 (DSPE)-PEG- COOH /卵磷脂、DSPE-PEG- COOH /DSPE-PEG- NH 2 的质量比以及 DSPE-PEG 端基的修饰。我们将模型化疗药物——亲水性顺铂 (DDP) 或疏水性 DDP 前药——封装于纳米颗粒 (NP) 中，结果显示其包封率高、稳定性佳、对高 FA 受体表达的 MCF-7 细胞具有特异性靶向识别能力，且 FA 受体表达量高，且细胞毒性较小。此类 PLGA-卵磷脂-PEG 核壳纳米颗粒 (NP) 已被证明是一种*具潜力的癌症靶向*药物递送纳米载体。

Abstract：

Protein cages have been widely investigated as molecular drug carrier. E2 protein from Bacillus stearothermophillus forms a dodecahedral cage structure of approximately 24 nm in diameter. To formulate a sustainable release profile, E2 protein was further encapsulated into poly(lactide-co-glycolide) (PLGA) microparticles to form a composite structure using water-in-oil-in-water (W/O/W) double emulsion method. The influence of fabrication parameters on microparticle morphology and E2 protein release profile were investigated. The microparticle size increased when the stirring speed of the second emulsification decreased. Decrease in the volume of external aqueous phase led to the reduction of microparticle size without affecting its porosity. The higher ionic concentration of external aqueous phase in the presence of surfactant resulted in microparticles with closed pores on surface. Increase in polymer concentration also led to the formation of less porous microparticles. The E2 protein was not dissociated upon encapsulation into PLGA microparticles based on the unchanged particle size of E2 protein. E2 protein release was studied in phosphate-buffered saline solution at 37°C. The initial burst and release rate were lowered as the surfactant concentration in external water phase during the fabrication process was increased from 0.1% to 1% (w/v). After 14-day incubation, no observable polymer degradation was found while the surface of microparticles appeared to be smoother than before incubation.

西安杏彩体育平台 生物提供相关产品：

VIP-PEG-DSPE

CREKA-PEG-DSPE

Asp8-PEG-DSPE

WYRGRL-PEG-DSPE

CTP-PEG-DSPE

CMP-PEG-DSPE

SHp-PEG-DSPE

CGKRK-PEG-DSPE

Xpeptide-PEG-DSPE

Asp6-PEG-DSPE

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