英文名称：pGBKT7-53 Vector 鲜酵母双改良品种差表媒体

其它的分类：pGBKT7-53 Control vector

规格为：2μl

输送管理温差：干冰输送管理

保存文档气温：-20℃存储

**期：1年

用：酒曲双杂交种

差不多消息承载类型的:酵母菌双杂交育种整体裁体

平台宽度:8.3kb

原核抗性:卡那霉素(Kanamycin, Kan )

克隆菌株:DH5a

形式标识c-Myc

需求标签:TRP1

发动子:ADH1

形容宿体:酒曲细胞核

5'测序引物:T7:TAATACGACTCACTATAGGG

3测序引物:跟据编码序列定制引物

质粒图谱：

描素∶

pGBKT7-53是弱阳對照质粒，能商品代码小鼠p53蛋清(a.a.72-390)和GAL4 DNA-BD(a.1-147)的就结合蛋白质。小鼠p53 cDNA克隆加入pGBKT7的EcoR和BanH1位点内。p53 cDNA起始于Lwabuchi et al. ( 1993 )年文献资料讲到的质粒（由Clontech整修所得的)。

存储与装卸搬运技术：

储存:-20°C保存图片，最少得1年**。运送:冰袋公路运输。

准备重大事项：

捷晨打造的质粒载体/质粒/菌株都经途认真的服务安全卫生性能调整。请收到货时后**行变为，再挑取单克隆扩张。车辆施用中若找到服务安全卫生性能间题，请和承堂迅速有效沟通。为了能够您的安全卫生和安全健康，请穿实验性服并戴一天性防护手套控制。

SD/-Ade with Agar 10×0.5L

SD/-Ade/-His with Agar 10×0.5L

SD/-Ade/-His/-Leu/-Met/-Trp with Agar 10×0.5L

SD/-Ade/-His/-Leu/-Met-Trp/-Ura with Agar 10×0.5L

SD/-Ade/-His/-Leu/-Trp with Agar 10×0.5 L Pouches

SD/-Ade/-His/-Leu/-Trp/-Ura with Agar 10×0.5L

SD/-Ade/-His/-Met with Agar 10×0.5L

SD/-Ade/-His/-Trp with Agar 10×0.5L Pouches

SD/-Ade/-Leu with Agar 10×0.5L

SD/-Ade/-Leu/-His with Agar 10×0.5L

SD/-Ade/-Leu/-Trp with Agar 10×0.5L

SD/-Ade/-Trp with Agar 10×0.5L Pouches

SD/-Arg/-His/-Leu/-Lys/-Ura with Agar 10×0.5L

SD/-Cys/-Met/-Ura with Agar 10×0.5L

SD/-Glu/-Ura with Agar 10×0.5L

SD/-His with Agar 10×0.5 L Pouches

SD/-His/-Leu with Agar 10×0.5 L Pouches

SD/-His/-Leu/-Met/-Trp with Agar 10×0.5 L Pouches

SD/-His/-Leu/-Met/-Ura with Agar 10×0.5L

SD/-His/-Leu/-Trp with Agar 10×0.5 L Pouches

SD/-His/-Leu/-Trp/-Ura with Agar 10×0.5 L Pouches

SD/-His/-Leu/-Ura with Agar 10×0.5 L Pouches

SD/-His/-Trp with Agar 10×0.5L Pouches

SD/-His/-Trp/-Ura with Agar 10×0.5 L Pouches

SD/-His/-Ura with Agar 10×0.5 L Pouches

SD/-Leu with Agar 10×0.5 L Pouches

SD/-Leu/-Ile/-Val with Agar 10×0.5L

SD/-Leu/-Met/-Trp with Agar 10×0.5L Pouches

SD/-Leu/-Met/-Trp/-Ura with Agar 10×0.5L

SD/-Leu/-Trp with Agar 10×0.5L Pouches

SD/-Leu/-Trp/-Ura with Agar 10×0.5L Pouches

SD/-Leu/-Ura with Agar 10×0.5L Pouches

SD/-Met with Agar 10×0.5L

SD/-Met/-Trp with Agar 10×0.5L Pouches

SD/-Trp with Agar 10×0.5L Pouches

SD/-Trp/-Ura with Agar 10×0.5L Pouches

SD/-Ura with Agar 10×0.5L Pouches